Hotspot

trnF-GAA-ndhJ

LSC · 48779-49371 · 593 bp

Back to Persicaria

Pi 0.0588

MarkerSeek score 80.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 593
variable_sites: 144
pi: 0.0588
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	AACAAGATGCAGGGTCGTTT	50.0	45.0	58.1	58.0	1104-1161 bp	1.0	91.4
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AAACAAGATGCAGGGTCGTT	50.0	45.0	58.1	58.0	1105-1162 bp	0.961538	89.9
trnF-GAA-ndhJ_p3	ATTGGGTCTCTTCCTCCACA	AACAAGATGCAGGGTCGTTT	50.0	45.0	58.0	58.0	1428-1476 bp	0.615385	76.1
trnF-GAA-ndhJ_p4	ATTGGGTCTCTTCCTCCACA	GGCTAATCAAACACGGGCTA	50.0	50.0	58.0	58.0	1400-1448 bp	0.615385	76.0
trnF-GAA-ndhJ_p5	ATTGGGTCTCTTCCTCCACA	AAACAAGATGCAGGGTCGTT	50.0	45.0	58.0	58.0	1429-1477 bp	0.576923	74.6