Hotspot

trnT-UGU-trnL-UAA

LSC · 46973-47737 · 765 bp

Back to Persicaria

Pi 0.0575

MarkerSeek score 82.90

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 765
variable_sites: 180
pi: 0.0575
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	441-849 bp	1.0	89.0
trnT-UGU-trnL-UAA_p2	GAGTCGAACCGATGACCATC	CCGTAGCGTCTACCGATTTC	55.0	55.0	58.2	58.2	472-880 bp	1.0	88.7
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	435-843 bp	1.0	87.7
trnT-UGU-trnL-UAA_p4	GAGTCGAACCGATGACCATC	CGTCTACCGATTTCGCCATA	55.0	50.0	58.2	57.6	466-874 bp	1.0	87.4
trnT-UGU-trnL-UAA_p5	CTAACCTCTGAGCTAAGCGG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.5	58.2	433-841 bp	1.0	86.0