Hotspot

rps4-trnT-UGU

LSC · 46259-46899 · 641 bp

Back to Persicaria

Pi 0.0685

MarkerSeek score 80.60

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 641
variable_sites: 178
pi: 0.0685
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	AATAGTCGGAGCCATACCCA	TTTCTCTTATGTGAGCCCGC	50.0	50.0	57.9	58.0	903-1080 bp	0.961538	89.5
rps4-trnT-UGU_p2	ATAGTCGGAGCCATACCCAA	TTTCTCTTATGTGAGCCCGC	50.0	50.0	57.9	58.0	902-1079 bp	0.961538	89.5
rps4-trnT-UGU_p3	TAGTCGGAGCCATACCCAAT	TTTCTCTTATGTGAGCCCGC	50.0	50.0	57.9	58.0	901-1078 bp	0.961538	89.5
rps4-trnT-UGU_p4	CCCCCAGACGTCGTATTTTT	TTTCTCTTATGTGAGCCCGC	50.0	50.0	57.9	58.0	649-826 bp	0.961538	89.4
rps4-trnT-UGU_p5	TAAAACCTGGCCTGTTGACC	TTTCTCTTATGTGAGCCCGC	50.0	50.0	58.0	58.0	887-1017 bp	0.884615	87.1