Hotspot

psbM-trnD-GUC

LSC · 29253-30086 · 834 bp

Back to Persicaria

Pi 0.0528
MarkerSeek score 80.10
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
834
variable_sites
188
pi
0.0528
unique_haplotype_count
21
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 CTGTTGATTCTAGCCTGCGT GCGTGCATCATCAATAAGCC 50.0 50.0 58.0 58.0 1626-1688 bp 0.269231 62.3
psbM-trnD-GUC_p2 ACTGTTGATTCTAGCCTGCG TGGAATTGGTTGTTCCTGGG 50.0 50.0 58.0 58.0 1595-1656 bp 0.153846 55.3
psbM-trnD-GUC_p3 CTGTTGATTCTAGCCTGCGT TGGAATTGGTTGTTCCTGGG 50.0 50.0 58.0 58.0 1594-1655 bp 0.153846 55.3
psbM-trnD-GUC_p4 ACTGTTGATTCTAGCCTGCG GGAATTGGTTGTTCCTGGGT 50.0 50.0 58.0 58.0 1594-1655 bp 0.153846 55.3
psbM-trnD-GUC_p5 CTGTTGATTCTAGCCTGCGT GGAATTGGTTGTTCCTGGGT 50.0 50.0 58.0 58.0 1593-1654 bp 0.153846 55.3