Hotspot

rpoB-trnC-GCA

LSC · 26615-27875 · 1261 bp

Back to Persicaria

Pi 0.0503

MarkerSeek score 82.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1261
variable_sites: 266
pi: 0.0503
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GTCGACATTCCCTCACTTCC	AAAGGATTTGCAGTCCTCCG	55.0	50.0	58.0	58.2	1010-1360 bp	1.0	90.5
rpoB-trnC-GCA_p2	GCCCAGGTATTGTCGACATT	AAAGGATTTGCAGTCCTCCG	50.0	50.0	58.0	58.2	1181-1371 bp	0.961538	88.8
rpoB-trnC-GCA_p3	TCGACATTCCCTCACTTCCA	AAAGGATTTGCAGTCCTCCG	50.0	50.0	58.4	58.2	1009-1359 bp	1.0	87.7
rpoB-trnC-GCA_p4	CATTCCCTCACTTCCAACCC	AAAGGATTTGCAGTCCTCCG	55.0	50.0	58.2	58.2	1331 bp	0.038462	38.9
rpoB-trnC-GCA_p5	CGACATTCCCTCACTTCCAA	AAAGGATTTGCAGTCCTCCG	50.0	50.0	57.5	58.2	1334 bp	0.038462	36.4