Hotspot

trnS-GCU-trnG-UCC

LSC · 8329-9089 · 761 bp

Back to Persicaria

Pi 0.0633

MarkerSeek score 80.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 761
variable_sites: 205
pi: 0.0633
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CACTCAGCCATCTCTCCCTA	ACGAATCACACTTTTACCACT	55.0	38.1	57.9	55.5	720-840 bp	1.0	63.1
trnS-GCU-trnG-UCC_p2	CACTCAGCCATCTCTCCCTA	AGAACGAATCACACTTTTACCAC	55.0	39.1	57.9	57.3	723-843 bp	1.0	62.0
trnS-GCU-trnG-UCC_p3	CACTCAGCCATCTCTCCCTA	GAACGAATCACACTTTTACCACT	55.0	39.1	57.9	57.3	722-842 bp	1.0	62.0
trnS-GCU-trnG-UCC_p4	CACTCAGCCATCTCTCCCTA	AACGAATCACACTTTTACCACT	55.0	36.4	57.9	56.2	721-841 bp	1.0	61.2
trnS-GCU-trnG-UCC_p5	CACTCAGCCATCTCTCCCTA	TACCACTAAACTATACCCGC	55.0	45.0	57.9	54.0	706-826 bp	1.0	59.3