Hotspot

psbK-psbI

LSC · 7637-8032 · 396 bp

Back to Persicaria

Pi 0.0619

MarkerSeek score 79.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 396
variable_sites: 96
pi: 0.0619
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TTACGTCCCGGGTCATTAGA	50.0	50.0	57.9	57.9	471-538 bp	1.0	90.4
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	ACGTCCCGGGTCATTAGATA	50.0	50.0	57.9	57.6	469-536 bp	1.0	88.6
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	TACGTCCCGGGTCATTAGAT	50.0	50.0	57.9	57.6	470-537 bp	1.0	88.6
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	TTACGTCCCGGGTCATTAGA	45.0	50.0	58.3	57.9	468-535 bp	1.0	88.4
psbK-psbI_p5	TTGGCAAGCTGCTGTAAGTT	TTACGTCCCGGGTCATTAGA	45.0	50.0	58.3	57.9	469-536 bp	1.0	88.4