Hotspot

ycf1

SSC · 128518-134082 · 5565 bp

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Pi 0.00881
MarkerSeek score 44.10
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5565
variable_sites
49
pi
0.00881
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 GAAAGGACCAATGTCTGTCGA AGAATGCTTGCCTAGAGTGT 47.6 45.0 58.0 56.5 232 bp 1.0 61.7
ycf1_p2 AGAAAGGACCAATGTCTGTCG AGAATGCTTGCCTAGAGTGT 47.6 45.0 58.0 56.5 233 bp 1.0 61.7
ycf1_p3 AAAGGACCAATGTCTGTCGA AGAATGCTTGCCTAGAGTGT 45.0 45.0 56.8 56.5 231 bp 1.0 60.3
ycf1_p4 GAAAGGACCAATGTCTGTCGA AAGAATGCTTGCCTAGAGTGT 47.6 42.9 58.0 57.3 233 bp 1.0 59.7
ycf1_p5 AGAAAGGACCAATGTCTGTCG AAGAATGCTTGCCTAGAGTGT 47.6 42.9 58.0 57.3 234 bp 1.0 59.7