Hotspot

trnL-UAG-ccsA

SSC · 118909-118996 · 88 bp

Back to Persea

Pi 0.046
MarkerSeek score 80.00
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
88
variable_sites
4
pi
0.046
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 TTATTTCGATGAAGCCGCCA AGTACTACGGTTTCATGGACT 45.0 42.9 58.0 56.1 290-291 bp 1.0 58.0
trnL-UAG-ccsA_p2 TAGACACGCTGCTCTTAGGA AGTACTACGGTTTCATGGACT 50.0 42.9 57.9 56.1 258-259 bp 1.0 57.4
trnL-UAG-ccsA_p3 AAATTGGTAGACACGCTGCT AGTACTACGGTTTCATGGACT 45.0 42.9 57.8 56.1 265-266 bp 1.0 56.9
trnL-UAG-ccsA_p4 GGTAGACACGCTGCTCTTAG AGTACTACGGTTTCATGGACT 55.0 42.9 57.8 56.1 260-261 bp 1.0 56.8
trnL-UAG-ccsA_p5 GTAGACACGCTGCTCTTAGG AGTACTACGGTTTCATGGACT 55.0 42.9 57.8 56.1 259-260 bp 1.0 56.8