Hotspot

ycf1-ndhF

SSC · 113879-113901 · 23 bp

Back to Persea

Pi 0.087
MarkerSeek score 71.80
Primer available yes
Label ycf1-ndhF

Feature Metadata

label_name
ycf1-ndhF
feature_type
igs
region
SSC
length_bp
23
variable_sites
2
pi
0.087
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-ndhF_p1 ACGAAGGGGACTAAACAGGA ACTTTGTTCGTTGGATCCGT 50.0 45.0 58.0 57.8 1154-1171 bp 1.0 78.5
ycf1-ndhF_p2 ACGAAGGGGACTAAACAGGA TCTGATGCGTTCCTGTAGGA 50.0 50.0 58.0 58.2 1260 bp 0.5 58.6
ycf1-ndhF_p3 ACAGGAACAAGAGGGATCCA TCTGATGCGTTCCTGTAGGA 50.0 50.0 58.0 58.2 1246 bp 0.5 58.4
ycf1-ndhF_p4 AAGAGGGATCCACCGAAGAA TCTGATGCGTTCCTGTAGGA 50.0 50.0 58.0 58.2 1238 bp 0.5 58.4
ycf1-ndhF_p5 TTTAGGCAGAATACCGTCGC TCTGATGCGTTCCTGTAGGA 50.0 50.0 58.1 58.2 1375 bp 0.5 58.2