Hotspot

rps11-rpl36

LSC · 83130-83241 · 112 bp

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Pi 0.0179
MarkerSeek score 62.80
Primer available yes
Label rps11-rpl36

Feature Metadata

label_name
rps11-rpl36
feature_type
igs
region
LSC
length_bp
112
variable_sites
2
pi
0.0179
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11-rpl36_p1 CGTCCATTCTTACGTGAACCA GTGAAAAATGTCGACTGATCCG 47.6 45.5 58.3 58.0 239 bp 1.0 54.0
rps11-rpl36_p2 CGTCCATTCTTACGTGAACC GTGAAAAATGTCGACTGATCCG 50.0 45.5 56.6 58.0 239 bp 1.0 53.1
rps11-rpl36_p3 CGTCCATTCTTACGTGAACCA ACATAAACAGAGACAGGGGT 47.6 45.0 58.3 55.7 178 bp 1.0 52.3
rps11-rpl36_p4 CGTCCATTCTTACGTGAACCA TGAAAAATGTCGACTGATCCGT 47.6 40.9 58.3 58.3 238 bp 1.0 51.4
rps11-rpl36_p5 CGTCCATTCTTACGTGAACCA TGAAAAATGTCGACTGATCCG 47.6 42.9 58.3 56.6 238 bp 1.0 51.3