Hotspot

psbE-petL

LSC · 68387-69690 · 1304 bp

Back to Persea

Pi 0.00872
MarkerSeek score 58.80
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1304
variable_sites
11
pi
0.00872
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA AAGCAGCCAGTAGAAAACCG 50.0 50.0 57.9 58.5 1413-1451 bp 1.0 78.8
psbE-petL_p2 CAACCCGCAATGAATAGGGA GCAGCCAGTAGAAAACCGAA 50.0 50.0 57.9 58.5 1411-1449 bp 1.0 78.8
psbE-petL_p3 ACGAATAACCAACCCGCAAT AAGCAGCCAGTAGAAAACCG 45.0 50.0 58.2 58.5 1422-1460 bp 1.0 77.8
psbE-petL_p4 ACGAATAACCAACCCGCAAT GCAGCCAGTAGAAAACCGAA 45.0 50.0 58.2 58.5 1420-1458 bp 1.0 77.8
psbE-petL_p5 TGCTGACGAATAACCAACCC AAGCAGCCAGTAGAAAACCG 50.0 50.0 58.2 58.5 1427-1465 bp 1.0 77.7