Hotspot

ycf4-cemA

LSC · 63693-64609 · 917 bp

Back to Persea

Pi 0.00657
MarkerSeek score 62.90
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
917
variable_sites
6
pi
0.00657
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 CATTCCCTTGACCCGTACTG AGATCCACCAGGGCAAAAAG 55.0 50.0 58.0 58.1 1086-1090 bp 1.0 80.0
ycf4-cemA_p2 CATTCCCTTGACCCGTACTG GAGATCCACCAGGGCAAAAA 55.0 50.0 58.0 58.1 1087-1091 bp 1.0 79.9
ycf4-cemA_p3 CATTCCCTTGACCCGTACTG GATCCACCAGGGCAAAAAGA 55.0 50.0 58.0 58.1 1085-1089 bp 1.0 79.9
ycf4-cemA_p4 ATTCCCTTGACCCGTACTGA AGATCCACCAGGGCAAAAAG 50.0 50.0 58.1 58.1 1085-1089 bp 1.0 79.7
ycf4-cemA_p5 ATTCCCTTGACCCGTACTGA GATCCACCAGGGCAAAAAGA 50.0 50.0 58.1 58.1 1084-1088 bp 1.0 79.7