Hotspot

accD-psaI

LSC · 61990-62589 · 600 bp

Back to Persea

Pi 0.012
MarkerSeek score 68.00
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
600
variable_sites
7
pi
0.012
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 AGCTACACGGTTTCTTTCCC GCCATTGCAATTGCTGGAAA 50.0 45.0 57.8 58.2 730-745 bp 1.0 79.4
accD-psaI_p2 GCTACACGGTTTCTTTCCCT GCCATTGCAATTGCTGGAAA 50.0 45.0 57.8 58.2 729-744 bp 1.0 79.4
accD-psaI_p3 AGCTACACGGTTTCTTTCCC AGAAGCCATTGCAATTGCTG 50.0 45.0 57.8 57.6 734-749 bp 1.0 77.9
accD-psaI_p4 GCTACACGGTTTCTTTCCCT AGAAGCCATTGCAATTGCTG 50.0 45.0 57.8 57.6 733-748 bp 1.0 77.9
accD-psaI_p5 GCTACACGGTTTCTTTCCCT TTGCTGGAAACACTAAGCCC 50.0 50.0 57.8 58.4 719-734 bp 1.0 77.9