Hotspot

trnE-UUC-trnT-GGU

LSC · 32994-33727 · 734 bp

Back to Persea

Pi 0.00694
MarkerSeek score 64.60
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
734
variable_sites
5
pi
0.00694
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CATGATCAACCCCCTTTCGT GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 884 bp 0.5 58.9
trnE-UUC-trnT-GGU_p2 CAACCCCCTTTCGTCTTACC GAACCGATGACTTACGCCTT 55.0 50.0 58.2 58.0 878 bp 0.5 58.5
trnE-UUC-trnT-GGU_p3 CATGATCAACCCCCTTTCGT CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 863 bp 0.5 58.5
trnE-UUC-trnT-GGU_p4 CATGATCAACCCCCTTTCGT TGGCGTTACTCTACCACTGA 50.0 50.0 57.9 58.1 860 bp 0.5 58.3
trnE-UUC-trnT-GGU_p5 CAACCCCCTTTCGTCTTACC CCATGGCGTTACTCTACCAC 55.0 55.0 58.2 58.1 857 bp 0.5 58.0