Hotspot

psbM-trnD-GUC

LSC · 31416-32396 · 981 bp

Back to Persea

Pi 0.00928
MarkerSeek score 63.80
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
981
variable_sites
9
pi
0.00928
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAACAACGCAGTAGCA AGTTCAATCGGTCAGAGCAC 42.9 50.0 58.2 57.9 1094-1128 bp 1.0 70.9
psbM-trnD-GUC_p2 GAATGAACAACGCAGTAGCA AGTTCAATCGGTCAGAGCAC 45.0 50.0 56.7 57.9 1093-1127 bp 1.0 70.7
psbM-trnD-GUC_p3 AGAATGAACAACGCAGTAGCA ATGCTGTACACCCCATTTCC 42.9 50.0 58.2 57.9 1123-1157 bp 1.0 70.4
psbM-trnD-GUC_p4 GAATGAACAACGCAGTAGCA ATGCTGTACACCCCATTTCC 45.0 50.0 56.7 57.9 1122-1156 bp 1.0 70.2
psbM-trnD-GUC_p5 GAATGAACAACGCAGTAGCAA AGTTCAATCGGTCAGAGCAC 42.9 50.0 57.5 57.9 1093-1127 bp 1.0 68.5