Hotspot

trnC-GCA-petN

LSC · 29107-30154 · 1048 bp

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Pi 0.00574
MarkerSeek score 62.30
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
1048
variable_sites
6
pi
0.00574
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTTGTATCGTTTTGGCGGC AGCCCAAGCGAGACTTACTA 45.0 50.0 57.9 58.2 1167-1170 bp 1.0 79.0
trnC-GCA-petN_p2 GGGTGTCGCCTGATTAACAA AGCCCAAGCGAGACTTACTA 50.0 50.0 58.2 58.2 1093-1096 bp 1.0 78.0
trnC-GCA-petN_p3 CTGCAAATCCTTTCTCCCCA AGCCCAAGCGAGACTTACTA 50.0 50.0 57.8 58.2 1123-1126 bp 1.0 77.7
trnC-GCA-petN_p4 TGCAAATCCTTTCTCCCCAG AGCCCAAGCGAGACTTACTA 50.0 50.0 57.8 58.2 1122-1125 bp 1.0 77.7
trnC-GCA-petN_p5 GCAAATCCTTTCTCCCCAGT AGCCCAAGCGAGACTTACTA 50.0 50.0 57.8 58.2 1121-1124 bp 1.0 77.7