Hotspot

atpF-atpH

LSC · 14363-14821 · 459 bp

Back to Persea

Pi 0.00885
MarkerSeek score 62.40
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
459
variable_sites
4
pi
0.00885
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 CCAGTGACCAAAGGAAACGA TCGTGGCATTAGCGCTTTTA 50.0 45.0 58.0 58.3 535-542 bp 1.0 79.2
atpF-atpH_p2 TTAAACCCGAAACTCCCAGC TCGTGGCATTAGCGCTTTTA 50.0 45.0 58.1 58.3 561-568 bp 1.0 78.6
atpF-atpH_p3 CCAGTGACCAAAGGAAACGA GATTTACGGACTGGTCGTGG 50.0 55.0 58.0 58.4 549-556 bp 1.0 78.4
atpF-atpH_p4 TTAAACCCGAAACTCCCAGC GATTTACGGACTGGTCGTGG 50.0 55.0 58.1 58.4 575-582 bp 1.0 77.8
atpF-atpH_p5 CCAGTGACCAAAGGAAACGA CGATTTACGGACTGGTCGTG 50.0 55.0 58.0 58.7 550-557 bp 1.0 75.4