Hotspot

trnK-UUU-rps16

LSC · 4412-5230 · 819 bp

Back to Persea

Pi 0.00489
MarkerSeek score 61.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
819
variable_sites
4
pi
0.00489
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 950-951 bp 1.0 78.9
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 949-950 bp 1.0 78.9
trnK-UUU-rps16_p3 AAAAGACCATAGCCGCACTT AAAAAGGCGCTCAACCTACA 45.0 45.0 57.8 58.0 971-972 bp 1.0 78.6
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA AAAAAGGCGCTCAACCTACA 50.0 45.0 58.3 58.0 959-960 bp 1.0 78.0
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT CTTGAAAAAGGCGCTCAACC 50.0 50.0 58.2 58.2 953-954 bp 1.0 77.2