Hotspot

rpl32-trnL-UAG

SSC · 104035-104563 · 529 bp

Back to Perotis

Pi 0.0277

MarkerSeek score 68.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 529
variable_sites: 26
pi: 0.0277
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ACCAAAAGGTTTTTCTGGGT	TCCTAAGAGCAGCGTGTCTA	40.0	50.0	55.5	57.9	516-590 bp	1.0	66.9
rpl32-trnL-UAG_p2	ACCAAAAGGTTTTTCTGGGT	AGCAGCGTGTCTACCAATTT	40.0	45.0	55.5	57.8	509-583 bp	1.0	66.5
rpl32-trnL-UAG_p3	ACCAAAAGGTTTTTCTGGGT	GCTTGAGCACTGCTTCCTAA	40.0	50.0	55.5	58.2	530-604 bp	1.0	66.4
rpl32-trnL-UAG_p4	ACCAAAAGGTTTTTCTGGGT	CCTAAGAGCAGCGTGTCTAC	40.0	55.0	55.5	57.8	515-589 bp	1.0	66.4
rpl32-trnL-UAG_p5	ACCAAAAGGTTTTTCTGGGT	CTAAGAGCAGCGTGTCTACC	40.0	55.0	55.5	57.8	514-588 bp	1.0	66.4