Hotspot

ndhC-trnV-UAC

LSC · 49747-50656 · 910 bp

Back to Perotis

Pi 0.0106

MarkerSeek score 66.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 910
variable_sites: 19
pi: 0.0106
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCTGGTCCTTCACTACTCGG	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.2	57.9	1048-1051 bp	1.0	79.5
ndhC-trnV-UAC_p2	TCTGGTCCTTCACTACTCGG	AGCCCAATGCAGAAAAATGC	55.0	45.0	58.2	57.9	1141-1144 bp	1.0	79.2
ndhC-trnV-UAC_p3	CACTACTCGGGGCTAAAAGC	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.3	57.9	1038-1041 bp	1.0	78.1
ndhC-trnV-UAC_p4	CACTACTCGGGGCTAAAAGC	AGCCCAATGCAGAAAAATGC	55.0	45.0	58.3	57.9	1131-1134 bp	1.0	77.7
ndhC-trnV-UAC_p5	TCTGGTCCTTCACTACTCGG	CGGTTCGAATCCGTATAGCC	55.0	55.0	58.2	58.3	1049-1052 bp	1.0	77.7