Hotspot

trnT-UGU-trnL-UAA

LSC · 45649-46459 · 811 bp

Back to Perotis

Pi 0.0181

MarkerSeek score 60.40

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 811
variable_sites: 27
pi: 0.0181
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.0	58.0	899-913 bp	1.0	84.2
trnT-UGU-trnL-UAA_p2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.1	58.0	891-905 bp	1.0	83.9
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	55.0	50.0	57.9	58.0	869-883 bp	1.0	83.4
trnT-UGU-trnL-UAA_p4	TCCTCGATAACGGGACATGA	ACAATCAAGTCCGTAGCGTC	50.0	50.0	57.9	58.0	1231-1242 bp	1.0	83.0
trnT-UGU-trnL-UAA_p5	ATTTGAACCGATGACCCTCG	GCTTCCATTGAGTCTCTGCA	50.0	50.0	58.0	57.9	1068-1082 bp	1.0	82.8