Hotspot

ycf3-trnS-GGA

LSC · 43734-44312 · 579 bp

Back to Perotis

Pi 0.0115

MarkerSeek score 62.70

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 579
variable_sites: 13
pi: 0.0115
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TCTCCTGAGGTTGTCGGAAT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.1	58.2	747-766 bp	1.0	81.2
ycf3-trnS-GGA_p2	TCTCCTGAGGTTGTCGGAAT	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.1	57.8	697-716 bp	1.0	81.1
ycf3-trnS-GGA_p3	TTCTCCTGAGGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	748-767 bp	1.0	79.4
ycf3-trnS-GGA_p4	TTTCTCCTGAGGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.3	58.2	749-768 bp	1.0	79.4
ycf3-trnS-GGA_p5	TTCTCCTGAGGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.3	57.8	698-717 bp	1.0	79.3