Hotspot

trnY-GUA-trnD-GUC

LSC · 14929-15282 · 354 bp

Back to Perotis

Pi 0.0237

MarkerSeek score 65.40

Primer available yes

Label trnY-GUA-trnD-GUC

Feature Metadata

label_name: trnY-GUA-trnD-GUC
feature_type: igs
region: LSC
length_bp: 354
variable_sites: 14
pi: 0.0237
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnD-GUC_p1	AATTCTTCCTGGGTCGATGC	GCGGTGCTCTAACCAATTGA	50.0	50.0	58.0	58.6	422-479 bp	1.0	81.7
trnY-GUA-trnD-GUC_p2	GGGGACGGACTGTAAATTCG	GCGGTGCTCTAACCAATTGA	55.0	50.0	58.4	58.6	389-446 bp	1.0	79.4
trnY-GUA-trnD-GUC_p3	GGGACGGACTGTAAATTCGT	GCGGTGCTCTAACCAATTGA	50.0	50.0	57.6	58.6	388-445 bp	1.0	79.2
trnY-GUA-trnD-GUC_p4	AATTCTTCCTGGGTCGATGC	GGCGGTGCTCTAACCAATT	50.0	52.6	58.0	58.1	423-480 bp	1.0	77.0
trnY-GUA-trnD-GUC_p5	TGGGGACGGACTGTAAATTC	GCGGTGCTCTAACCAATTGA	50.0	50.0	57.2	58.6	390-447 bp	1.0	76.0