Hotspot

psbI-trnS-GCU

LSC · 7595-7714 · 120 bp

Back to Perotis

Pi 0.0181

MarkerSeek score 62.30

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 120
variable_sites: 4
pi: 0.0181
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	AATCCTGGACGTGAGGAGTA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.5	58.0	209 bp	1.0	77.0
psbI-trnS-GCU_p2	ATCCTGGACGTGAGGAGTAA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.5	58.0	208 bp	1.0	77.0
psbI-trnS-GCU_p3	TAATCCTGGACGTGAGGAGT	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.5	58.0	210 bp	1.0	77.0
psbI-trnS-GCU_p4	ATCCTGGACGTGAGGAGTAA	CTTTCGATTTGGAACGGGGA	50.0	50.0	57.5	58.2	245 bp	1.0	75.6
psbI-trnS-GCU_p5	ATCCTGGACGTGAGGAGTAA	TTTCGATTTGGAACGGGGAG	50.0	50.0	57.5	58.2	244 bp	1.0	75.6