Hotspot

ndhA

SSC · 119405-121605 · 2201 bp

Back to Periploca

Pi 0.00963

MarkerSeek score 50.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2201
variable_sites: 51
pi: 0.00963
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CGTGAGACAGGGTAATCGTG	AGAGTTGAAGCCCCAAAAGG	55.0	50.0	58.1	58.0	2584-2593 bp	1.0	81.3
ndhA_p2	TATGTATCGGGCAGCTCGTA	AGAGTTGAAGCCCCAAAAGG	50.0	50.0	58.1	58.0	2553-2562 bp	1.0	81.3
ndhA_p3	CGTGAGACAGGGTAATCGTG	TCCAACCTTTGAATTGGCGA	55.0	45.0	58.1	58.0	2621-2630 bp	1.0	81.2
ndhA_p4	CGTGAGACAGGGTAATCGTG	GTGAGAGTTGAAGCCCCAAA	55.0	50.0	58.1	58.0	2587-2596 bp	1.0	81.2
ndhA_p5	TATGTATCGGGCAGCTCGTA	TCCAACCTTTGAATTGGCGA	50.0	45.0	58.1	58.0	2590-2599 bp	1.0	81.1