Hotspot

ndhF-rpl32

SSC · 112633-113322 · 690 bp

Back to Periploca

Pi 0.0143

MarkerSeek score 62.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 690
variable_sites: 24
pi: 0.0143
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGTATGAGCCACCCGTATTG	CGCTGTCCAATATCCCTTCC	55.0	55.0	58.1	58.1	809-847 bp	1.0	81.3
ndhF-rpl32_p2	ATAGGAACGGGAAGTGGGAT	CGCTGTCCAATATCCCTTCC	50.0	55.0	57.8	58.1	833-871 bp	1.0	80.7
ndhF-rpl32_p3	GAACGGGAAGTGGGATGAAA	CGCTGTCCAATATCCCTTCC	50.0	55.0	57.8	58.1	829-867 bp	1.0	80.7
ndhF-rpl32_p4	AAGGTATGAGCCACCCGTAT	CGCTGTCCAATATCCCTTCC	50.0	55.0	58.2	58.1	811-849 bp	1.0	80.7
ndhF-rpl32_p5	AGGTATGAGCCACCCGTATT	CGCTGTCCAATATCCCTTCC	50.0	55.0	58.2	58.1	810-848 bp	1.0	80.7