Hotspot

psbF-psbE

LSC · 64605-64613 · 9 bp

Back to Periploca

Pi 0.0444
MarkerSeek score 65.30
Primer available yes
Label psbF-psbE

Feature Metadata

label_name
psbF-psbE
feature_type
igs
region
LSC
length_bp
9
variable_sites
1
pi
0.0444
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbF-psbE_p1 GAACAGCCAACCATCGTACT AACTGGCCGTTTTGATCCTT 50.0 45.0 57.9 58.0 116 bp 1.0 79.9
psbF-psbE_p2 GAACAGCCAACCATCGTACT ACTGGCCGTTTTGATCCTTT 50.0 45.0 57.9 58.0 115 bp 1.0 79.9
psbF-psbE_p3 AACAGCCAACCATCGTACTG AACTGGCCGTTTTGATCCTT 50.0 45.0 58.2 58.0 115 bp 1.0 79.1
psbF-psbE_p4 AACAGCCAACCATCGTACTG ACTGGCCGTTTTGATCCTTT 50.0 45.0 58.2 58.0 114 bp 1.0 79.1
psbF-psbE_p5 TGAACAGCCAACCATCGTAC ACTGGCCGTTTTGATCCTTT 50.0 45.0 58.2 58.0 116 bp 1.0 79.1