Hotspot

psaI-ycf4

LSC · 59829-60264 · 436 bp

Back to Periploca

Pi 0.0119

MarkerSeek score 55.70

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 436
variable_sites: 13
pi: 0.0119
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	TTTCCGGCAATTGCAATGTC	GGATAAAGGCCCAGCAGAAA	45.0	50.0	57.9	57.9	573-578 bp	1.0	82.0
psaI-ycf4_p2	TTTCCGGCAATTGCAATGTC	AAAGGATAAAGGCCCAGCAG	45.0	50.0	57.9	57.9	576-581 bp	1.0	81.9
psaI-ycf4_p3	TAGGCCTAGTATTTCCGGCA	GGATAAAGGCCCAGCAGAAA	50.0	50.0	57.9	57.9	584-589 bp	1.0	81.8
psaI-ycf4_p4	TAGGCCTAGTATTTCCGGCA	AAAGGATAAAGGCCCAGCAG	50.0	50.0	57.9	57.9	587-592 bp	1.0	81.7
psaI-ycf4_p5	GGCCTAGTATTTCCGGCAAT	GGATAAAGGCCCAGCAGAAA	50.0	50.0	57.7	57.9	582-587 bp	1.0	80.4