Hotspot

rpoB-trnC-GCA

LSC · 26798-28010 · 1213 bp

Back to Periploca

Pi 0.0166

MarkerSeek score 51.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1213
variable_sites: 49
pi: 0.0166
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GAAAAGACCGGATCTCCACC	GGGAAATACTTCGGCGAGTT	55.0	50.0	58.0	58.0	2070-2079 bp	0.8	72.2
rpoB-trnC-GCA_p2	CGAGCCCATATCCTTGCTTT	GGGAAATACTTCGGCGAGTT	50.0	50.0	58.0	58.0	1863-1872 bp	0.8	72.2
rpoB-trnC-GCA_p3	GAAAAGACCGGATCTCCACC	CGCTTCTGCTGGGAAATACT	55.0	50.0	58.0	58.0	2080-2089 bp	0.8	72.1
rpoB-trnC-GCA_p4	CGAGCCCATATCCTTGCTTT	CGCTTCTGCTGGGAAATACT	50.0	50.0	58.0	58.0	1873-1882 bp	0.8	72.1
rpoB-trnC-GCA_p5	ACCGAAATTCCGCTATGGTC	GGGAAATACTTCGGCGAGTT	50.0	50.0	58.1	58.0	1785-1794 bp	0.8	72.1