Hotspot

atpH-atpI

LSC · 13393-14596 · 1204 bp

Back to Periploca

Pi 0.00797

MarkerSeek score 56.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1204
variable_sites: 24
pi: 0.00797
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	GGCTTATATCGGCGAATCCA	45.0	50.0	58.0	57.6	1151-1273 bp	1.0	82.2
atpH-atpI_p2	TACCCTCTACAGCTTGACCC	GGCTTATATCGGCGAATCCA	55.0	50.0	58.1	57.6	1234-1356 bp	1.0	81.3
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1170-1292 bp	1.0	81.1
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	GGCTTATATCGGCGAATCCA	45.0	50.0	58.2	57.6	1152-1274 bp	1.0	81.0
atpH-atpI_p5	TACCCTCTACAGCTTGACCC	TTTTTGCAACTTTAGCCGCG	55.0	45.0	58.1	58.5	1253-1375 bp	1.0	80.2