Hotspot

trnK-UUU-rps16

LSC · 4198-4852 · 655 bp

Back to Periploca

Pi 0.0128

MarkerSeek score 55.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 655
variable_sites: 20
pi: 0.0128
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCGATGGTATGGACGAATCC	GCCGTACGAGGAGAAAACTT	55.0	50.0	57.9	57.9	1046-1156 bp	1.0	81.0
trnK-UUU-rps16_p2	TGGATACCTCGGAAACAGGT	GCCGTACGAGGAGAAAACTT	50.0	50.0	58.1	57.9	1588-1699 bp	1.0	80.9
trnK-UUU-rps16_p3	TGGATACCTCGGAAACAGGT	ACTGGGCCAGACTTAAGGAT	50.0	50.0	58.1	58.0	1880-1960 bp	0.8	71.8
trnK-UUU-rps16_p4	CCGATGGTATGGACGAATCC	ACTGGGCCAGACTTAAGGAT	55.0	50.0	57.9	58.0	1338-1418 bp	0.8	71.6
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	ACTGGGCCAGACTTAAGGAT	50.0	50.0	58.2	58.0	1276-1356 bp	0.8	70.9