Hotspot

trnH-GUG-psbA

LSC · 77-349 · 273 bp

Back to Periploca

Pi 0.0178

MarkerSeek score 61.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 273
variable_sites: 11
pi: 0.0178
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	55.0	55.0	58.1	58.3	369-391 bp	1.0	82.6
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	383-405 bp	1.0	82.5
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	382-404 bp	1.0	82.5
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	384-406 bp	1.0	82.4
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	55.0	50.0	58.1	57.6	370-392 bp	1.0	82.1