Hotspot

ndhE

SSC · 116337-116642 · 306 bp

Back to Perilla

Pi 0

MarkerSeek score 20.50

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 20.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	ATACGCTTGGGCCAGAAAAA	TTCATCTTTGGACCGTTGGG	45.0	50.0	58.1	58.1	1400 bp	1.0	78.9
ndhE_p2	GACCCATACTGCGAGTTGTT	TTCATCTTTGGACCGTTGGG	50.0	50.0	57.9	58.1	1275 bp	1.0	78.7
ndhE_p3	CTAATACATCGGTGGGGCAC	TTCATCTTTGGACCGTTGGG	55.0	50.0	58.1	58.1	1119 bp	1.0	78.6
ndhE_p4	ACCTCTTACAACCAACGCAG	TTCATCTTTGGACCGTTGGG	50.0	50.0	58.1	58.1	1200 bp	1.0	78.5
ndhE_p5	ATACGCTTGGGCCAGAAAAA	GTCTTTCTAGGACCGGGTCT	45.0	55.0	58.1	58.2	1632 bp	1.0	78.4