Hotspot

ndhF-rpl32

SSC · 111570-111987 · 418 bp

Back to Perilla

Pi 0.00399
MarkerSeek score 42.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
418
variable_sites
5
pi
0.00399
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAACGAAAGGT GCCCCATAGCCTTTCCTTTT 50.0 50.0 57.7 58.1 550 bp 1.0 77.8
ndhF-rpl32_p2 CTGGAAGTGGAACGAAAGGT AAGAAAACGCCTTTAACGCC 50.0 45.0 57.7 57.6 570 bp 1.0 75.5
ndhF-rpl32_p3 CTGGAAGTGGAACGAAAGGT CCCATAGCCTTTCCTTTTCCA 50.0 47.6 57.7 57.9 548 bp 1.0 70.0
ndhF-rpl32_p4 CCACACACGACGAAGATTTT GCCCCATAGCCTTTCCTTTT 45.0 50.0 56.7 58.1 618 bp 1.0 69.1
ndhF-rpl32_p5 CTGGAAGTGGAACGAAAGGT AAAGAAAACGCCTTTAACGCC 50.0 42.9 57.7 58.3 571 bp 1.0 68.7