Hotspot

rpl20-rps12

LSC · 68812-69629 · 818 bp

Back to Perilla

Pi 0.00122

MarkerSeek score 44.10

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 818
variable_sites: 3
pi: 0.00122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	ACCCTCGAAAACTTGATGCA	CGCCGAGGAACATGTACTAG	45.0	55.0	57.7	57.9	924-925 bp	1.0	77.1
rpl20-rps12_p2	CGGGCTATATATCCGCGTTT	CGCCGAGGAACATGTACTAG	50.0	55.0	57.7	57.9	877-878 bp	1.0	77.0
rpl20-rps12_p3	ACCCTCGAAAACTTGATGCA	AATACAAGACAGCCCGTTCG	45.0	50.0	57.7	58.3	984-985 bp	1.0	75.9
rpl20-rps12_p4	CGGGCTATATATCCGCGTTT	AATACAAGACAGCCCGTTCG	50.0	50.0	57.7	58.3	937-938 bp	1.0	75.7
rpl20-rps12_p5	GTTCTACGTTTCCGGGCTAT	CGCCGAGGAACATGTACTAG	50.0	55.0	57.4	57.9	889-890 bp	1.0	74.6