Hotspot

accD-psaI

LSC · 58744-59470 · 727 bp

Back to Perilla

Pi 0.00138

MarkerSeek score 44.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 727
variable_sites: 3
pi: 0.00138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TAAGCTCCACGCCTTCTTTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	843 bp	1.0	79.3
accD-psaI_p2	CTCCACGCCTTCTTTCCTTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	839 bp	1.0	79.3
accD-psaI_p3	TAAGCTCCACGCCTTCTTTC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.9	57.9	849 bp	1.0	78.7
accD-psaI_p4	CTCCACGCCTTCTTTCCTTT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.1	57.9	845 bp	1.0	78.6
accD-psaI_p5	CGACTTAATCGTACCGCGTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.6	58.0	894 bp	1.0	77.1