Hotspot

trnT-GGU-psbD

LSC · 31158-32465 · 1308 bp

Back to Perilla

Pi 0.000255

MarkerSeek score 43.50

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1308
variable_sites: 1
pi: 0.000255
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1456-1459 bp	1.0	77.7
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	CCCTCCGTAACCAATCATCC	55.0	55.0	58.1	57.7	1440-1443 bp	1.0	77.6
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1459-1462 bp	1.0	77.5
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	CCCTCCGTAACCAATCATCC	50.0	55.0	58.1	57.7	1443-1446 bp	1.0	77.4
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	GCCAGACCAGCCTACAAAAA	55.0	50.0	58.1	58.4	1469-1472 bp	1.0	76.6