Hotspot

petN-psbM

LSC · 28460-29490 · 1031 bp

Back to Perilla

Pi 0.00097

MarkerSeek score 43.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1031
variable_sites: 3
pi: 0.00097
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	AGTTCCGACCGCTTTTCTAC	50.0	50.0	58.0	57.9	1120 bp	1.0	79.6
petN-psbM_p2	ACTCGTAGTGTGGGGAAGAA	GTTCCGACCGCTTTTCTACT	50.0	50.0	58.0	57.9	1119 bp	1.0	79.6
petN-psbM_p3	TCGTAGTGTGGGGAAGAAGT	AGTTCCGACCGCTTTTCTAC	50.0	50.0	58.0	57.9	1118 bp	1.0	79.6
petN-psbM_p4	TCGTAGTGTGGGGAAGAAGT	GTTCCGACCGCTTTTCTACT	50.0	50.0	58.0	57.9	1117 bp	1.0	79.6
petN-psbM_p5	CGTAGTGTGGGGAAGAAGTG	GTTCCGACCGCTTTTCTACT	55.0	50.0	57.9	57.9	1116 bp	1.0	79.0