Hotspot

trnS-GCU-trnG-UCC

LSC · 8408-9099 · 692 bp

Back to Perilla

Pi 0.000963
MarkerSeek score 43.90
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
692
variable_sites
2
pi
0.000963
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 762 bp 1.0 70.3
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 761 bp 1.0 70.3
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 762 bp 1.0 70.2
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 761 bp 1.0 70.2
trnS-GCU-trnG-UCC_p5 ACGGAAAGAGAGGGATTCGA ACGAATCGCACTTTTACCACT 50.0 42.9 58.2 58.2 816 bp 1.0 69.1