Hotspot

rps16-trnQ-UUG

LSC · 5965-7001 · 1037 bp

Back to Perilla

Pi 0.00161

MarkerSeek score 44.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1037
variable_sites: 5
pi: 0.00161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1116-1118 bp	1.0	79.8
rps16-trnQ-UUG_p2	GTCTTTCAAGTCGCACGTTG	GTCTCGCTATTCGGAGGTTC	50.0	55.0	58.0	57.9	1129-1131 bp	1.0	79.4
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1109-1111 bp	1.0	78.7
rps16-trnQ-UUG_p4	AATCCACAATGGGTCGTGTC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1133-1135 bp	1.0	78.4
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GTCTCGCTATTCGGAGGTTC	45.0	55.0	58.1	57.9	1122-1124 bp	1.0	78.3