Hotspot

petL-psaJ

LSC · 66991-67870 · 880 bp

Back to Pericopsis

Pi 0.00859

MarkerSeek score 61.70

Primer available yes

Label petL-psaJ

Feature Metadata

label_name: petL-psaJ
feature_type: igs
region: LSC
length_bp: 880
variable_sites: 7
pi: 0.00859
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-psaJ_p1	AAGCCGCTAGTAGAAAACCA	AAGCCCCGAACCATAGAGTA	45.0	50.0	56.9	57.8	939-987 bp	1.0	74.1
petL-psaJ_p2	AGCCGCTAGTAGAAAACCAA	AAGCCCCGAACCATAGAGTA	45.0	50.0	56.9	57.8	938-986 bp	1.0	74.1
petL-psaJ_p3	AAGCCGCTAGTAGAAAACCA	ATCAAGAGACCCGCTAAAGC	45.0	50.0	56.9	57.7	955-1003 bp	1.0	72.9
petL-psaJ_p4	AGCCGCTAGTAGAAAACCAA	ATCAAGAGACCCGCTAAAGC	45.0	50.0	56.9	57.7	954-1002 bp	1.0	72.9
petL-psaJ_p5	AGCCGCTAGTAGAAAACCAA	GCTAAAGCCCCGAACCATAG	45.0	55.0	56.9	58.4	942-990 bp	1.0	72.2