Hotspot

petA-psbJ

LSC · 63208-64495 · 1288 bp

Back to Pericopsis

Pi 0.00396

MarkerSeek score 62.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1288
variable_sites: 5
pi: 0.00396
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTGTCGAAGGTCTCTTGTT	CATATTCCGGATTGGGCTCA	50.0	50.0	57.9	57.4	1411-1428 bp	1.0	76.1
petA-psbJ_p2	CGTTACGTGTCGAAGGTCTC	CATATTCCGGATTGGGCTCA	55.0	50.0	58.4	57.4	1416-1433 bp	1.0	74.0
petA-psbJ_p3	CGTGTCGAAGGTCTCTTGTT	TATTCCGGATTGGGCTCATC	50.0	50.0	57.9	57.1	1409-1426 bp	1.0	73.7
petA-psbJ_p4	CGTGTCGAAGGTCTCTTGTT	TCATATTCCGGATTGGGCTC	50.0	50.0	57.9	57.1	1412-1429 bp	1.0	73.7
petA-psbJ_p5	GATCCGTTACGTGTCGAAGG	CATATTCCGGATTGGGCTCA	55.0	50.0	58.5	57.4	1420-1437 bp	1.0	73.5