Hotspot

rps16-accD

LSC · 57384-58099 · 716 bp

Back to Pericopsis

Pi 0.00699

MarkerSeek score 62.90

Primer available yes

Label rps16-accD

Feature Metadata

label_name: rps16-accD
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 5
pi: 0.00699
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-accD_p1	AACAAGGCGCTCAACCTAC	TGGTCTTCACTTACACTGGT	52.6	45.0	58.1	56.0	948-950 bp	1.0	56.1
rps16-accD_p2	GAACAAGGCGCTCAACCTAC	TGGTCTTCACTTACACTGGT	55.0	45.0	59.2	56.0	949-951 bp	1.0	55.0
rps16-accD_p3	AACAAGGCGCTCAACCTAC	CCACACCTGTATTCTAACTCCC	52.6	50.0	58.1	57.9	888-890 bp	1.0	54.9
rps16-accD_p4	AACAAGGCGCTCAACCTAC	CCCACACCTGTATTCTAACTCC	52.6	50.0	58.1	57.9	889-891 bp	1.0	54.9
rps16-accD_p5	GAACAAGGCGCTCAACCTA	TGGTCTTCACTTACACTGGT	52.6	45.0	57.8	56.0	949-951 bp	1.0	54.8