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psbK-trnQ-UUG

LSC · 55215-55688 · 474 bp

Back to Pericopsis

Pi 0.00654

MarkerSeek score 62.90

Primer available yes

Label psbK-trnQ-UUG

Feature Metadata

label_name: psbK-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 474
variable_sites: 3
pi: 0.00654
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnQ-UUG_p1	AGGCTTCAGGTAATTTCGCC	GATATGCTCTGGGACGGAAG	50.0	55.0	58.2	57.6	628-642 bp	1.0	76.7
psbK-trnQ-UUG_p2	AGGCTTCAGGTAATTTCGCC	GACCAAAACCCGTTGCCTTA	50.0	50.0	58.2	58.7	586-600 bp	1.0	75.0
psbK-trnQ-UUG_p3	GGCTTCAGGTAATTTCGCCA	GATATGCTCTGGGACGGAAG	50.0	55.0	58.5	57.6	627-641 bp	1.0	74.4
psbK-trnQ-UUG_p4	AGGCTTCAGGTAATTTCGCC	AATGGATATGCTCTGGGACG	50.0	50.0	58.2	57.2	632-646 bp	1.0	73.5
psbK-trnQ-UUG_p5	AGGCTTCAGGTAATTTCGCC	GAATAGCGGGACCAAAACCC	50.0	55.0	58.2	58.9	595-609 bp	1.0	73.2