Hotspot

trnR-UCU-trnG-GCC

LSC · 52398-52715 · 318 bp

Back to Pericopsis

Pi 0.0279

MarkerSeek score 72.10

Primer available yes

Label trnR-UCU-trnG-GCC

Feature Metadata

label_name: trnR-UCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 318
variable_sites: 8
pi: 0.0279
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnG-GCC_p1	AGAAGACCTCTGTCCTATCCA	AGCCTTCCAAGCTAACGATG	47.6	50.0	56.9	58.0	367-398 bp	1.0	69.0
trnR-UCU-trnG-GCC_p2	AGAAGACCTCTGTCCTATCCA	CCTAGCCTTCCAAGCTAACG	47.6	55.0	56.9	58.1	370-401 bp	1.0	68.8
trnR-UCU-trnG-GCC_p3	AGGTTTAGAAGACCTCTGTCCT	AGCCTTCCAAGCTAACGATG	45.5	50.0	57.9	58.0	373-404 bp	1.0	68.3
trnR-UCU-trnG-GCC_p4	AGGTTTAGAAGACCTCTGTCCT	CCTAGCCTTCCAAGCTAACG	45.5	55.0	57.9	58.1	376-407 bp	1.0	68.1
trnR-UCU-trnG-GCC_p5	AGAAGACCTCTGTCCTATCCA	CCCTAGCCTTCCAAGCTAAC	47.6	55.0	56.9	57.7	371-402 bp	1.0	66.6