Hotspot

psbM-petN

LSC · 32120-32923 · 804 bp

Back to Pericopsis

Pi 0.00871

MarkerSeek score 63.80

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 804
variable_sites: 7
pi: 0.00871
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	CTCGCGTTTATTGCTACTGC	GGGCCGCTTTAATGGTAGTT	50.0	50.0	57.6	58.2	962-965 bp	1.0	75.8
psbM-petN_p2	CTCGCGTTTATTGCTACTGC	CTTGGGCCGCTTTAATGGTA	50.0	50.0	57.6	58.2	965-968 bp	1.0	75.8
psbM-petN_p3	CTCGCGTTTATTGCTACTGC	TTGGGCCGCTTTAATGGTAG	50.0	50.0	57.6	58.2	964-967 bp	1.0	75.8
psbM-petN_p4	CTCGCGTTTATTGCTACTGC	ATGGGGAAGAAGTGGACTCT	50.0	50.0	57.6	57.6	916-919 bp	1.0	75.0
psbM-petN_p5	CTCGCGTTTATTGCTACTGC	CCCTCGTAGTATGGGGAAGA	50.0	55.0	57.6	57.6	926-929 bp	1.0	74.8