Hotspot

ndhF

SSC · 109405-111648 · 2244 bp

Back to Periandra

Pi 0.0169

MarkerSeek score 52.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2244
variable_sites: 38
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TTCCTTCTTCGAATTCGGGC	AGGAATTTGGTTATTGGTCCA	50.0	38.1	58.3	55.1	2795-2838 bp	1.0	47.5
ndhF_p2	TGGGTTTATTACGGGACAGC	AGGAATTTGGTTATTGGTCCA	50.0	38.1	57.6	55.1	2736-2779 bp	1.0	46.4
ndhF_p3	GGGACAGCTCATGATGTTCA	AGGAATTTGGTTATTGGTCCA	50.0	38.1	57.6	55.1	2724-2767 bp	1.0	46.4
ndhF_p4	CGATCATTGTGGTCGGACTC	AGGAATTTGGTTATTGGTCCA	55.0	38.1	58.4	55.1	2860-2903 bp	1.0	46.1
ndhF_p5	TTCCTTCTTCGAATTCGGGC	ACTAGGAATTTGGTTATTGGTCCA	50.0	37.5	58.3	57.9	2798-2841 bp	1.0	45.9