Hotspot

ndhA

SSC · 118648-120969 · 2322 bp

Back to Periandra

Pi 0.019

MarkerSeek score 61.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2322
variable_sites: 44
pi: 0.019
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCATAAGGAACATATCGTGA	TGGGAGAAGTTGATCGTTGA	38.1	45.0	54.2	56.5	2436-2447 bp	1.0	41.8
ndhA_p2	TGAACCTAGTTACCATAAGGAACA	TGGGAGAAGTTGATCGTTGA	37.5	45.0	57.1	56.5	2447-2458 bp	1.0	41.8
ndhA_p3	ACCATAAGGAACATATCGTGAA	TGGGAGAAGTTGATCGTTGA	36.4	45.0	55.0	56.5	2436-2447 bp	1.0	41.8
ndhA_p4	ACCATAAGGAACATATCGTGA	TGGGAGAAGTTGATCGTTGAA	38.1	42.9	54.2	57.2	2436-2447 bp	1.0	41.8
ndhA_p5	TGAACCTAGTTACCATAAGGAACA	TGGGAGAAGTTGATCGTTGAA	37.5	42.9	57.1	57.2	2447-2458 bp	1.0	41.8