Hotspot

ndhE-ndhG

SSC · 116882-117137 · 256 bp

Back to Periandra

Pi 0.048

MarkerSeek score 70.00

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 256
variable_sites: 12
pi: 0.048
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TTCGGCTTGTGATCAATCCA	TTGTCGTGGCTCGTCAATAA	45.0	45.0	57.5	57.6	346-352 bp	1.0	76.7
ndhE-ndhG_p2	TCGGCTTGTGATCAATCCAT	TTGTCGTGGCTCGTCAATAA	45.0	45.0	57.3	57.6	345-351 bp	1.0	74.9
ndhE-ndhG_p3	TTCGGCTTGTGATCAATCCA	ATAGGTGCAATTGTCGTGGC	45.0	50.0	57.5	58.9	356-362 bp	1.0	72.9
ndhE-ndhG_p4	TTCGGCTTGTGATCAATCCAT	TTGTCGTGGCTCGTCAATAA	42.9	45.0	58.0	57.6	346-352 bp	1.0	72.6
ndhE-ndhG_p5	GGCTCTAACCATATTTCGGCT	TTGTCGTGGCTCGTCAATAA	47.6	45.0	57.9	57.6	360-366 bp	1.0	71.4